Figure 5
Detection of lamins A and C, lamin B1 and emerin by Western blotting in fibroblasts from the subject with HGPS. Fibroblasts from unaffected controls (lanes 1 and 2) and the subject with HGPS (lanes 3 and 4) were lysed in Laemmli buffer, and whole-cell extracts corresponding to 0.5 × 106 cells (lanes 1 and 3) and 0.25 × 106 (lanes 2 and 4) were analyzed by immunoblotting using anti-lamin A/C (lane: Lamin A/C), anti-lamin B1 (lane: Lamin B1) and anti-emerin (lane: Emerin) antibodies. Arrowhead points to the position of the prelamin A mutant in lanes 3 and 4.