Figure 1

Hinderin: Genomic organization, structural domains, and expression pattern in human tissues. A) The sequence of the Hinderin ORF was used to BLAST the human genome database. The numeration of the exon boundaries is relative to the transcriptional start site. The size of the intronic sequences is based on the numeration of the human contiguous sequences. B) Northern blot hybridization of Hinderin in HeLa and HCT116 cells. A single main transcript of ~ 4.5 kb is identifiable. C) Prediction of coiled-coil domains in Hinderin. The numbers on the abscissa corresponds to the amino acid residues. The probability of the polypeptide to assume a coiled coil conformation is plotted on the ordinate axis. The contribution of the different exons to the five-domain structural organization of Hinderin is also illustrated. The sequence encoded by exons 3 through 6, bears a 36% homology to the SMC protein consensus sequence. A bipartite nuclear localization signal is harbored in exon 7. D) Expression of Hinderin in different human tissues. PCR amplification was stopped after 30 cycles and the product analyzed on 2% agarose staining with ethidium bromide. The G3PDH transcript was amplified in 20 cycles and used to show uniformity of the source cDNA in the different specimens.