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Table 1 Quantitation of immunogold label in normal melanocytes transfected with GFP-HPS3 or GFP-HPS3-delCBD1

From: Association of the Hermansky-Pudlak syndrome type-3 protein with clathrin

 

Gold Particles

Clathrin Association

Intracellular compartment

GFP-HPS3

GFP-HPS3-delCBD

GFP-HPS3

GFP-HPS3-delCBD

Small vesicles in Golgi area 2,3

57

8

53

1

Golgi stacks

1

1

0

0

Other small vesicles

15

16

9

3

Endosomal structures 3

23

17

15

1

Mitochondria

2

2

0

0

Melanosomes

3

2

0

0

Nucleus

2

1

0

0

Cytoplasm 2

8

63

0

3

Total 3

111

110

77

8

  1. 1 Anti-GFP immunogold particles were counted in a total of 28 GFP-HPS3 and 25 GFP-HPS3-delCBD expressing cells
  2. 2 Difference in number of gold particles per compartment, for GFP-HPS3-delCBD compared with GFP-HPS3, is statistically significant by Chi square analysis (p < 0.001)
  3. 3 Difference in number of gold particles found in association with clathrin, for GFP-HPS3-delCBD compared with GFP-HPS3, is statistically significant by Chi square analysis (p < 0.001)
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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