Figure 12

Immunostaining of MPRO cells with various HP1 antibodies. Panels: A, undifferentiated; B, granulocyte forms of MPRO. Columns: FITC, antibody staining viewed by conventional epifluorescent microscopy; DAPI (DNA staining). Cells were stained with anti-HP1 α, β and γ. Note the co-localization of anti-HP1 γ with DAPI-bright pericentric heterochromatin. Panel C: confocal images demonstrating co-localization of anti-trimethylated H4K20 with anti-HP1 γ stained granulocyte pericentric heterochromatin. Scale bars: 10 μm.