Granulocyte heterochromatin: defining the epigenome

Table 1 Summmary Of Granulocyte Immunochemistry*.

ANTIBODY	HUMAN BLOOD GRANULOCYTES	MOUSE BLOOD GRANULOCYTES	MOUSE MPRO CELLS**
dimethyl H3K9	+ N	+ N	+ N
trimethyl H3K9	+ N g	+ N p	+ N p
monomethyl H3K27	+ N	+ N	+ N
dimethyl H3K27	+ N	+ N	+ N
trimethyl H3K27	+ N	+ N	+ N
dimethyl H4K20	+/- N, C	+/- N	+/- N
trimethyl H4K20	+ N, C	+ N p	+ N p
HP1 α	0^‡	0	+/- N, C
HP1 β	0^‡	0	+/- N, C
HP1 γ	+/- N, C	+ N p	+ N p

*The results listed represent a qualitative assessment, combining data from immunostaining and immunoblotting. In the case of normal mouse blood, due to inadequate enrichment of granulocytes, no immunoblotting was performed.
** Identical immunostaining and immunoblotting results were obtained with undifferentiated and RA differentiated MPRO cells.
Abbreviations: N, nuclear staining; N g, nuclear coarse granules; N p, nuclear pericentric heterochromatin; N, C, nuclear and cytoplasmic staining.
Reaction intensity: +, moderate to strong; +/-, weak; 0, negligible.
^‡ Moderate to strong staining in the cytoplasm only.

ISSN: 2661-8850