Figure 4

Immunolocalization of MFP labels faint filaments that co-localize with cortical MTs, but does not label mitotic MT structures. Indirect immunofluorescence analysis of a fixed onion epidermal cell probed with affinity purified rabbit anti-MFP antibodies (A) and mouse anti-tubulin antibodies (B) and visualized by epifluorescence microscopy. An overlay (C) shows the co-localization of MT labeling by the two antibodies (arrowheads). (D) Fluorescence immunostaining of MT structures with a tubulin antibody in four stages of mitosis in Arabidopsis suspension cells labels the pre-prophase band, the mitotic spindle in metaphase and anaphase, and the phragmoplast in telophase. The MFP antibody labels peroxisomes but not these MT structures in dividing cells. DNA was stained with DAPI (blue). Bars, 6 μm.