Figure 3

Inhibiting GSK3β with lithium chloride enhances Notch signals in primary human CD4⁺, but not Jurkat, T-cells. (A) Primary human CD4⁺ T-cells were nucleofected with 4 μg p10xCBF-luc, 0.5 μg pTK-RL and 1 μg of either pN1-IC or pcDNA3.1 and plated o/n in triplicate with increasing concentrations of LiCl. Data is presented as the fold induction in normalised luminescence compared to the untreated vector only control. (B) Primary human CD4⁺ T-cells were nucleofected with 4 μg p10xCBF-luc and exposed to different levels of plate-bound Delta1-Fc in the presence or absence of LiCl (10 mM). (C) J-N2 cells were transfected with p10xCBF1-luc and pTK-RL and stimulated overnight in increasing concentrations of LiCl. Data is presented as the fold induction in normalised luminescence compared to the untreated IgG4 control.