Figure 10
From: Microtubules regulate disassembly of epithelial apical junctions
Association of kinesin-1 with AJ proteins. T84 cell lysates were immunoprecipitated with either anti-kinesin-1 heavy chain antibody, anti-kinesin-2 heavy chain antibody, or control mouse IgG and immunoblotted for different junctional proteins as described in the Methods section. Kinesin-1 co-precipitates with AJ proteins E-cadherin, β-catenin and p120 catenin, but not with TJ proteins occludin and JAM-A or desmosomal component desmoglein-2. No co-pecipitation of any tested junctional protein with either kinesin-2 or control IgG is seen.