Figure 11

Thalidomide attenuates NO mediated migration in ECs: Scratching the EC monolayer with a sterile plastic scraper, a linear wound was made. The cells were incubated with 500 μmol SNP for 1 hour. The SNP treated cells were treated with thalidomide (25, 50, and 75 μg/ml) and incubated for 8 hours. Images were taken at 0 and 8 hours after adding thalidomide to the cells. Quantification of the areas of the wounds was performed by using Scion Image, Release Alpha 4.0 3.2 and Adobe Photoshop version 6.0. The data depicts that rate of SNP mediated wound healing were slower by 50% and 85% in 50 and 75 μg/ml thalidomide treated cells respectively in comparison to that of control. Cells treated with 25 μg/ml thalidomide showed no significant change in SNP mediated wound healing. The asterisks represent the level of significance. * P < 0.05 compared to control.