Thalidomide impairs basal wound healing in EC monolayer. Wound was created with a sterile plastic scraper and images were taken at 0 and 8 hours. A representative image from top panel shows how the proliferation of ECs heal the wound in 8 hours. The rate of wound healing was quantified (in the graph below) from the images using Scion Image, Release Alpha 4.0 3.2 and Adobe Photoshop version 6.0. Analysis of the data depicts that the rate of wound healing is slower in 100 and 150 μg/ml thalidomide treated cells. The asterisks represent the level of significance. * P < 0.05 compared to control.