Table 1 List of primers used in this study
From: Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)
Primer sets | Sequences 5'→3' | Predicted PCR product size (bp) | Tm (°C) | References |
|---|---|---|---|---|
IAP hu Fo | CCG-CTT-TAA-CCA-GTG-CAA-CA | 60 | ||
452 | - | |||
IAP hu Ba | CCC-ATG-AGA-TGG-GTC-ACA-GA | 62 | ||
MUC5AC Fo | TGA-TCA-TCC-AGC-AGC-AGG-GT | 62 | ||
408 | [25] | |||
MUC5AC Ba | CCG-AGC-TCA-GAG-GAC-ATA-TGG-G | 70 | ||
bACT hu Fo | AGA-AAA-TCT-GGC-ACC-ACA-CC | 60 | ||
485 | - | |||
bACT hu Ba | GTC-AGG-CAG-CTC-GTA-GCT-CT | 64 |