Skip to main content

Table 1 List of primers used in this study

From: Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)

Primer sets Sequences 5'→3' Predicted PCR product size (bp) Tm (°C) References
IAP hu Fo CCG-CTT-TAA-CCA-GTG-CAA-CA   60  
   452   -
IAP hu Ba CCC-ATG-AGA-TGG-GTC-ACA-GA   62  
MUC5AC Fo TGA-TCA-TCC-AGC-AGC-AGG-GT   62  
   408   [25]
MUC5AC Ba CCG-AGC-TCA-GAG-GAC-ATA-TGG-G   70  
bACT hu Fo AGA-AAA-TCT-GGC-ACC-ACA-CC   60  
   485   -
bACT hu Ba GTC-AGG-CAG-CTC-GTA-GCT-CT   64