Table 1 Primary antibodies, dilutions, and sources of antibodies used in immunohistochemical study
Antibody | Clone | Dilution | Pretreatment | Source/Country |
|---|---|---|---|---|
ER | 6F-11 | 1:100 | Steamear/citrate | Novocastra/UK |
PR | PgR 312 | 1:100 | Steamear/citrate | Novocastra/UK |
p53 | DO-7 | 1:500 | Steamear/citrate | Dakocytomation/USA |
p21 | 4D10 | 1:20 | Steamear/citrate | Novocastra/UK |
p63 | 4A4 | 1:100 | Steamear/EDTA | Dakocytomation/USA |
Ki-67 | MIB-1 | 1:100 | Steamear/citrate | Immunotech/France |