rad24+ was required for normal cytoplasmic accumulation of Rad26-GFP after 20 minutes of MBC treatment. Cultures of rad26-gfp (TE1197), rad25Δ rad26-gfp (TW1237) and rad24Δ rad26-gfp (TW1238) in liquid EMM minimal media were left untreated or treated with MBC for 20 minutes. The figure was made by merging DIC and GFP images. Arrows point to untreated rad25Δ rad26-gfp cells that have cytoplasmic Rad26-GFP signal. Notice that rad24Δ cells are more spherical than rad24+ and rad25Δ cells. The percentage of cells containing cytoplasmic Rad26-GFP signal is shown (N > 100). Bar = 5 μm.