Figure 3

CEES reduces intracellular NO in LPS stimulated RAW264.7 macrophages. Panel A: Intracellular DCFH (20 μM) oxidation in LPS stimulated macrophages (as indicated) incubated for 2 h. Fluorescence (excitation 485 nm, emission 520 nm) was measured in Relative Fluorescence Units (RFU); the oxidation rate was expressed as RFU/min. Panel B: Macrophages stimulated with 20 ng/ml LPS, were incubated in the presence or absence of 500 μM CEES (as indicated) for 24 h. Post, CEES was applied after the 24 hours of LPS stimulation; Sim, CEES was applied simultaneously with LPS. Panel C: LPS stimulated cells were incubated in the presence or absence of 25 μM ebselen, a selective iNOS inhibitor (as indicated). 10, 10 ng/ml LPS; 20, 20 ng/ml LPS. Mean values not sharing a common letter are significantly different (p < 0.05).