Figure 4

Subcellular targeting of the I/LWEQ modules of Talin-a and Talin-b. HeLa cells were transiently transfected with either the dsRed-Talin-a.2341–2531 or the dsRed-Talin-b.2341–2531 fusion construct (I/LWEQ module) and counterstained with vinculin to independently label focal adhesions or fluorescein-phalloidin to label F-actin, as previously described [13]. The Talin-a I/LWEQ module targeted to focal adhesions, where the fluorescence signals for dsRed-Talin-a.2341–2531 and the focal adhesion component vinculin overlap (Column 1, overlay). The Talin-a I/LWEQ module did not preferentially localize to actin stress fibers (Column 2). In contrast to Talin-a, the Talin-b I/LWEQ module was not targeted to focal adhesions (Column 3). However, dsRed-Talin-b.2341–2531 did colocalize with actin stress fibers, as shown by the colocalization of the fluorescence signals of dsRed-Talin-b.2341–2531 with phalloidin-stained F-actin (Column 4, overlay).