Figure 6

Role of Cdk-5 in the regulation of AAH. SH-Sy5y cells were transfected with recombinant plasmids to over-express Luciferase (pLuc) as a negative control, Cdk-5, p35, p25, Cdk-5+p35, Cdk-5+p25, or pAAH. The cells were harvested 48 hours later to measure (A) Cdk-5 activity, (B) luciferase activity, (C) AAH immunoreactivity by Western blot analysis with b-actin loading control and (D) digital imaging of the Western blot results, (E) AAH mRNA with levels normalized to 18S, and (F) directional motility using the ALMI assay. The graphs depict the mean ± S.D. of results from 6 replicate independent cultures. Inter-group comparisons were made using ANOVA with post hoc Fisher LSD tests (*P < 0.001 and +P < 0.01 relative to pLuc-transfected control cells).