Figure 2

The N-terminal deletion mutant is not regulated by Bcl-2 in Drosophila melanogaster. A, Cell lysates of S2 cells transfected with UAS-GFP (lane 1), UAS-Bax-GFP (lane 2) or UAS-Bax 30–192-GFP (lane 3) along with actin-GAL4 for 24 hours were assessed by western blot analysis using an anti -GFP antibody. B, Viability of flies was assessed in the indicated UAS transgenic lines driven at 25°C by vg-GAL4 (left panel) or twist-GAL4 (right panel). Viability is represented as the percentage of eclosed adult flies expressing Bax-GFP or Bax 30–192-GFP with respect to the appropriate sibling controls. The numbers over each bar indicate the total number of flies screened in the group. C-H, Wing phenotypes in vg-GAL4 flies sibling controls (C); transgenic fly lines of UAS-Bax-GFP (D); UAS-Bax 30–192-GFP (E); Bcl-2 (F); Bax-GFP + Bcl-2 (G) or Bax 30–192-GFP + Bcl-2 (H). I, Wing phenotypes of flies expressing Bax-GFP or Bax 30–192-GFP were categorized as illustrated. Representative images are shown. Arrow indicates notched margin of the wing obtained in few animals.