Figure 4
Bcl-x L regulation of colocalization with Bid and Bim in HEK cells. A and B, HEK cells transfected with Bax or Bax and Bcl-xL were stained for Bid or Bim. C and D, As in A, except that cells were transfected with Bax 30–192 with or without Bcl-xL. E and F, Cells transfected with Bax 30–105 with and without Bcl-xL were stained with clone 6A7 and Bid or Bim. Nuclear morphology was assessed by staining with Hoechst 33342. Single confocal planes of cells imaged at 60× magnification are shown. Scale bar: 10 microns.