Figure 3

Effect of hook proteins on CFTR distribution. Hook proteins and CFTR were co-expressed in Vero cells for 36 hours in the absence (A) or presence (B) of 10 μM lactacystin for the final 12 hours. Graphs show the fraction of cells with aggregated CFTR (top) or with CFTR in juxtanuclear aggresomes (bottom), as shown in Figure 2A. In the rest of cells, CFTR was distributed diffusely throughout the cell. At least 400 cells were counted for each construct in at least two separate assays. For hook2 and hook2 truncations more than 5000 cells from at least ten separate assays were counted and yielded similar results. Results shown are from one representative experiment in which all constructs were used in parallel. While absolute numbers changed significantly in different experiments, the relative effect of different constructs was highly consistent between different experiments.