Figure 2
From: Absence of gemin5 from SMN complexes in nuclear Cajal bodies
Gemins 3, 4, 6 and 7 colocalise with SMN in nuclear gems/CBs, but gems/CBs in most cells lack gemin5. HeLa cells grown on coverslips were fixed with 1% formalin in PBS and permeabilized with 1% Triton X-100. Gemins 4, 5, 6 and 7 were identified using GEM4E, GEM5Q, GEM6F and GEM7E, respectively in a double label with SMN rabbit antiserum. Typical fields are shown, except that an unusual field containing several cells with gemin5-positive gems/CBs was chosen for GEM5Q. The anti-gemin3 rabbit antiserum was double-labelled with MANSMA1 mAb against SMN. Alexa fluor 546 goat anti-mouse IgG (red) and Alexa fluor 488 goat anti-rabbit IgG (green) were used as second antibodies. DAPI (blue) was used to counterstain the nuclei. (See text for white and green arrows).