Figure 4
From: Absence of gemin5 from SMN complexes in nuclear Cajal bodies
Overexpression of SMN in HeLa cells stimulates formation of gems/CBs containing other gemins, except gemin 5. Gemin2 (A), gemin 4 (B), gemin6 (D) and 7 (E) co-localise with SMN in HeLa cells stably transfected with human SMN1 in pcDNA3 [49]. Gemin5 (C) does not co-localise with SMN. The same mAbs as in Fig. 2 were used, plus MANSIP1A for gemin2. Double label of SMN mAb MANSMA1 with gemin3 rabbit antiserum (F), unrip rabbit antiserum (G) and fibrillarin human auto-antiserum (H), or SMN antiserum with p80 mAb (I) were performed on the HeLa cell line stably transfected with SMN1. Alexa fluor 546 goat anti-mouse IgG (red) was used to detect mouse mAbs and Alexa fluor 488 goat anti-rabbit IgG (green) was used to detect rabbit antibodies. FITC-conjugated goat anti-human IgG (Chemicon) was used to detect fibrillarin. DAPI (blue) was used to counterstain the nuclei.