Table 3 Oligonucleotide primers for PCR
From: Absence of gemin5 from SMN complexes in nuclear Cajal bodies
Primer | Primer sequences (5'-3') |
|---|---|
Gemin6F | gag gat cc c atg agt gaa tgg atg aag |
Gemin6R | gcc tcg ag t cat tgg gaa gct gta aga |
Gemin7F | gag gat cc a atg caa act cca gtg aac |
Gemin7R | gcc tcg ag t tat ggc ttg aag gta ta |
Gemin3F | agt gac cta ctg ttg ccg g |
Gemin3R | cat ctt cct ggg ctt cag tgc |
Gemin 4F | atc gcg aat tc c atg gac cta gga ccc ttg |
Gemin 4R | ata att ctc gag cag cac ctc gtc tgg ctc |
Gemin 5FN | gca ctg gat cc t cga gtc ata gga gag ttg |
Gemin 5RN | gct cgg aag ctt cca tac acg gat cat gcc |
Gemin 5FC | aat tag gat cc g atg gcc tcc tgg gac gag |
Gemin 5RC | gcg cgg aag ctt cat aca gaa ggt ctg gca |