Figure 1From: Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegansAll human EHD proteins rescue the vacuolated intestinal phenotype in the intestine of C. elegans rme-1 ( b1045 ). (A) Micrograph images of middle intestinal regions of transgenic animals expressing the human EHD proteins. The rme-1(b1045) worms were injected with pVha-6::SL2-GFP (50 ng/μl) or with the same construct containing the human EHD cDNAs along with myo2::GFP (100 ng/μl) as a co-injection marker. Intestinal vacuoles are viewed as spaces devoid of green fluorescence in the rme-1(b1045) mutant (arrows). (B) Intestinal vacuoles were counted in at least 3 independent transgenic lines expressing no vector (rme-1(b1045)), vector alone (Vector), or a vector containing EHD1-4. (C) Basolateral endocytosis assay of the intestinal vacuoles. Adult hermaphrodites were microinjected with 1 mg/mL Texas-Red BSA (TR-BSA) into the pseudocoelom and examined for uptake in intestinal vacuoles. Lack of accumulation of TRed-BSA microinjected into the pseoudocelum in wild-type (WT) worms (N2 Bristol strain) (left). Rapid accumulation of TR-BSA in the enlarged intestinal vacuoles (arrows) in the rme-1(b1045) mutant worms (middle). rme-1(b1045) worms rescued with human EHD4 do not display accumulation of the dye in any intestinal cells (right) similar to WT animals. * – pseudocoelom, ** – gonads. DIC – differential interference contrast microscopy.Back to article page