Table 1 List of proteins purified from hydra lysates by 14-3-3-affinity chromatography
identification | protein score (matched peptides) | size of hydra protein (kDa) | size of gel band (kDa) | 14-3-3 binding motif | previously identified 14-3-3 target |
|---|---|---|---|---|---|
cytoskeleton | |||||
tubulin α | 102 (6) | 53 | 53 | no | [32] |
tubulin β | 89 (5) | 50 | 50 | yes | [32] |
actin | 186 (8) | 41 | 40 | yes | [27, 32] |
tropomyosin | 101 (7) | 27 | 37 | yes | [32] |
calcium signalling | |||||
calmodulin | 71 (6) | 17 | 17 | no | [30, 32] |
calcium binding prot. | 134 (9) | 20 | 20 | no | |
calcium adaptor AIF-1 | 112 (7) | 17 | 17 | no | |
metabolism | |||||
fru-1,6-BP-aldolase | 98 (6) | 36 | 41 | no | |
GAPDH | 172 (9) | 36.5 | 37 | yes (3) | [26, 28, 32] |
PEPCK | 53 (3) | 71 (hs) | 64 | yes (hs) | |
ATP-synthase β | 217 (13) | 56 | 50 | yes (2) | [25, 32] |
protein biosynthesis/-folding | |||||
EF-2 | 51 (3) | 97 | 96 | yes | [32] |
EF1 α | 156 (8) | 51 | 50 | yes (2) | [32] |
eIF-3 β | 132 (5) | 36 | 36 | yes (2) | |
Hsp70 | 174 (10) | 75 | 70 | yes | [29, 31, 32] |
PDI-like protein | 78 (6) | 48 (dm) | 50 | yes | |
Miscellaneous | |||||
ppod3 | 212 (10) | 30 | 30 | no | |
ppod4 | 290 (8) | 32 | 32 | yes | |
14-3-3 HyA | 157 (6) | 28.6 | 30 | - | |
14-3-3 HyB | 187 (8) | 28.1 | 30 | - | |
unknown protein | 51 (4) | 19 | 32 | no | |
hypothetical protein | 95 (6) | 24 | 80 | no |