Figure 1

Domain organisation of HS1^-/- and genotyping of knockout mice. (A) Schematic representation of mouse cortactin and HS1 proteins. N – terminal acidic domain, R1, R2, etc – Cortactin repeats, CC – coiled coil helical domain, PRD – proline rich domain, SH3 – C-terminal Src homology domain. Numbers indicate amino acid number. (B) Genotyping of HS1 knockout mice by PCR. WT – wild type, HS1^+/- – heterozygote, HS1^-/- – homozygote. (C) Western blot of platelet extracts from WT and HS1^-/- mice probed with α-HS1 (top panel) and α-tubulin (bottom panel).