Figure 3
From: Regulation of tight junction assembly and epithelial morphogenesis by the heat shock protein Apg-2
Retardation of ZO-1 recruitment to forming junctions by Apg-2 depletion. Control and Apg-2 RNAi cell lines were cultured in low calcium with tetracycline and junction formation was then induced for the indicated periods of time before fixation. The cells were then labelled with antibodies against Apg-2 (A), ZO-1 (B), or fluorescent phalloidin (C). If tetracycline was not added and Apg-2 was not depleted, Apg-2 RNAi cell lines formed junctions at the same speed as control RNAi cell lines (not shown). Note, recruitment of ZO-1 to the forming junctional complex was retarded, but the distribution of ZO-1 appeared to be normal at later time points.