Figure 3

Mobility shift of endogenous Borealin. Cell lysates of asynchronously growing and mitotic Hela and WT-8 cells were separated using 12.6% SDS-polyacrylamide gel and assessed by Western blotting (WB) using antibodies to endogenous (end) Borealin or the Flag-tag. Transfers were stripped and reprobed for β-actin as a loading control.