Effects of siRNA-AdipoR1 transfection or AICAR treatments on Osterix mRNA expression in MC3T3-E1 cells. Total RNA was collected at 4, 7, 10 days after siRNA-AdipoR1 transfection. Real-time PCR was performed as described in MATERIALS AND METHODS. Osterix mRNA expressions was suppressed by blocking the receptor expression (A). AICAR (0.5 mM), an AMP kinase stimulator, was added after the cells reached confluency. Total RNA was collected at 14, 21, 28 days. Osterix mRNA expressions was enhanced by AICAR (B). The result was the representative of five different experiments.