Figure 2

Co-localization of GFP-ML1 with soluble endocytosed molecules. A) Confocal images of stable GFP-ML1 (green) cells that endocytosed BSA-AlexaFluor 594 (red) for 1 minute and then chased for the indicated times before fixation. The bottom panel of the 15-minute time point represents a magnification of the area indicated in the top panel. Bar is 5 μm in unmagnified panels. B) Quantitation of the extent of co-localization of BSA-AlexaFluor 594 with GFP-ML1 at various chase times. Bars represent standard deviations. C) Confocal images of stable GFP-ML1 (green) cells that endocytosed Dextran-Rhodamine (red) for 1 minute and then chased for the indicated times before fixation. Some chase times are not shown. The bottom panel of the 15-minute time point represents an artificial magnification of the area indicated in the top panel. Bar is 5 μm in unmagnified panels. D) Quantitation of the extent of co-localization of Dextran-Rhodamine with GFP-ML1 at various chase times. Bars represent standard deviations.