Differences in fusion protein localization during pseudopod formation. GFP-FLN (A-E) and GFP-FLNABD (F-J) showed similar patterns of localization during pseudopod formation. Both probes maintain a cortical localization as the new protrusion begins to develop between the plasma membrane and the actin cortex (A and F) as the developing protrusion matures the cortex eventually breaks down and a new cortex forms at the periphery of the new protrusion (E and J). GFP-αA (K-N) and GFP-αAABD (O-R) showed no localization to the actin cortex during or after maturation of the pseudopod. Both probes remain cytoplasmic until the protrusion starts (K and O) and remains localized to the actin within the protrusion as the protrusion grows (L-N and P-R).