Figure 7From: Application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of Influenza A virus strainExpression analysis of construct lacking obpNLS and construct with obpNLS in fusion with a known cytoplasmic protein. NPΔ obpNLS, transfected in COS-7L cells, exhibits a characteristic cytoplasmic localization pattern. C2-GFP, used as a vector control, localizes to the cytoplasm. Rotavirus non structural protein 6 (NSP6) fused with GFP (GFP-NSP6) exhibits a typical punctate, cytoplasmic localization pattern. However, note that the obpNLS+NSP6 (obpNLS fused to the N-terminus of NSP6) exhibit an exclusive nuclear localization pattern. Bar represents 20 μm.Back to article page