Figure 4

CORT-induced nuclear translocation of NFAT2 in mLTC-1 cells. mLTC-1 cells were transiently transfected with NFAT2-GFP expressing vector. 36 h after transfection, cells were treated with 100 nM CORT or 100 nM CORT plus 100 ng/ml CsA for 12 h. The cells were rinsed with PBS and fixed. Representative fields of cells were viewed by Confocal microscopy and photographed.