Figure 1

NAC effect on viability and NO production in CEES/LPS treated RAW 264.7 cells (simultaneous NAC/CEES/LPS application). Panel A: Macrophages incubated with 50 or 100 ng/ml of LPS or/and 500 μM CEES were simultaneously treated with or without 10 mM NAC (as indicated) for 24 hours. Cell viability was measured using the MTT assay (see Materials and Methods) and expressed as OD at 575 nm. Panel B: Macrophages were incubated as described above and NO production measured as the concentration of nitrite in the culture media as described in Materials and Methods. Mean values not sharing a common letter are significantly different (p < 0.05).