ILK localization is altered with kindlin-2 knockdown. A. Immunoblot on protein from C2C12 cells at differentiation day 2 (D2) and day 4 (D4) transfected with scrambled (scr) or kindlin-2 (kind2) RNAi. Blots were probed with anti-ILK and anti-GAPDH. No difference by densitometry was detected between scrambled and kindlin-2 RNAi cells. Dot indicates ILK band. B. Immunoblot on subcellular fractions from day 2 differentiated C2C12 cells probed with anti-ILK. ILK localizes to the cytosolic (cyto) fraction in scrambled RNAi cells and to the membrane/insoluble (mem) fraction in kindlin-2 RNAi cells. No expression was seen with either condition in the cytoskeletal or nuclear fractions (data not shown).