FET gene expression is attenuated in differentiating human embryonic stem cells. (a) A colony of spontaneously differentiating hESCs. Inner and peripheral parts of the colonies were harvested based on cell morphology. (b) Relative expression of selected mRNAs in samples from inner or peripheral parts of colonies analyzed by quantitative real-time PCR with ACTB as endogenous control. POU5F1 – pluripotency marker,SOX2 – ectodermal marker, VIM – mesodermal marker,CCNA2 – proliferation marker. The day 12 measurement consists of one sample taken from inseparable, mixed cell populations. (c) Undifferentiated hESCs show positive FET protein staining. Cells were stained with primary antibodies for FET proteins and visualized with Cy3-conjugated secondary antibodies (red). The merge images additionally show DAPI staining of nuclei (blue).