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Figure 4 | BMC Cell Biology

Figure 4

From: Importin alpha binding and nuclear localization of PARP-2 is dependent on lysine 36, which is located within a predicted classical NLS

Figure 4

Lysine 36 but not lysine 37 of PARP-2 is critical for nuclear localization. A) HA-tagged wild type (wt) PARP-2 or the indicated single mutants were expressed in HEK293T cells and expression was analyzed by western blot using a monoclonal anti-HA antibody. 50 μg of whole cell extracts were used, endogenous PARP-1 levels served as loading control. B) HEK293T cells were transfected with HA-tagged wild type (wt) PARP-2 or with the PARP-2 mutants K36R and K37R. HA-tagged proteins were detected by immunofluorescence as described for Figure 2A. Representative images are presented.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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