Figure 4

Effect of wortmannin (WM) on insulin-mediated colocalization of remodeled actin, Glut-4 molecule in C2Glut-4^wt/+FAK^wt/+ cells. Serum starved (4 h) C2Glut-4^wt/+FAK^wt/+ myotubes were differentiated under MF and MFI condition and stimulated with insulin (100 nM) for 30 min. Cells were preincubated with 200 nM WM for 30 min, followed by insulin-stimulation (100 nM) for 30 min under MF and MFI condition. Cells were fixed, permeabilized, stained with Phalloidin Texas Red and nucleus was stained with DAPI. Images were captured from different fields and a representative image of 3 experiments is presented. Bar represents 5 μm. a: Cells differentiated under MF condition stimulated with insulin; b: Cells differentiated under MF condition, treated with WM and stimulated with insulin; c: Cells differentiated under MFI condition stimulated with insulin; d: Cells differentiated under MFI condition, treated with WM and stimulated with insulin. White arrow head represent actin filaments, Yellow arrow head represent Glut-4 molecules and Blue arrow head indicates colocalized actin with Glut-4.