Figure 1

Schematic representation of the FIT approach. Removal of the substrate binding domains (SH2 and SH3) of NTKs (denoted as kinase domain) abolishes the association with and tyrosine phosphorylation of substrates (denoted as S1–S5). Transfection of FIT-compatible kinase and a FIT-compatible substrate (possessing artificial binding interface; represented by wavy line) promotes specific association between the kinase and substrate leading to its tyrosine phosphorylation (denoted by P) and hence downstream effect(s).