Figure 3
From: Phosphorylation of p130Cas initiates Rac activation and membrane ruffling
FIT-mediated specific phosphorylation of p130Cas in SYF cells leads to increased tyrosine phosphorylation of p130Cas at focal adhesions. SYF cells were transfected either with vectors expressing GFP-actin, FIT-compatible Src and FIT-compatible p130Cas (A) or with GFP-actin, ΔSrc (lacking substrate binding domains) and p130Cas, both lacking the artificial binding interface. Tyrosine phosphorylation was visualized by anti-pTyr and rhodamine while p130Cas localization was visualized by anti-Cas and Alexa-647. Merged image is presented on right. Scale bars represent 10 μM.