Figure 6

Ik2 protein is co-localized with spn-F in the nurse cells and at the anterior ring of the oocyte. (A) Live imaging of stage 9 egg chamber from nanos-Gal4; pUASP GFP-Ik2 transgenic flies. Ik2 is localized to the anterior end of the oocyte. (B-D) Confocal image of stage 8 egg chamber from nanos-Gal4; pUASP GFP-Ik2 transgenic flies stained with anti-Spn-F. (B) GFP-Ik2 in green; (C) immunostaining for Spn-F in red; (D) merged picture. Both proteins are co-localized along the anterior cortex of the oocyte and in a punctate pattern in the nurse cells. Egg chambers are positioned that the anterior (A) is to the left left and posterior end (P) is to the right.