Figure 3

Localization of p36-CAT fusion proteins in BY-2 cells. (A) Schematic illustrations of CAT and various p36-CAT fusion proteins and their corresponding subcellular localizations in transformed (via biolistic bombardment) BY-2 cells. The numbers in the name of each fusion construct denote the specific amino acid residues from p36 that were fused to the N terminus of CAT. Portions of the p36 ORF are colored blue or black, the latter denoting the two putative TMDs in p36 and the numbers shown above p36-CAT include the position of each TMD's left and right amino acid border. Grey boxes denote CAT. Mito, mitochondria; Cyt, cytosol. (B) Representative (immuno)epifluorescence micrographs illustrating the localizations of the various constructs shown in (A). Each micrograph is labelled at the top left with the name of the transiently-expressed fusion protein or the endogenous mitochondrial marker protein, E1β. Hatched boxes represent the portion of the cells shown at higher magnification in the panels to the right. Arrowheads indicate obvious colocalizations. Bar = 10 μm.