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Figure 5 | BMC Cell Biology

Figure 5

From: Phosphatidylinositol 3-kinase signaling in proliferating cells maintains an anti-apoptotic transcriptional program mediated by inhibition of FOXO and non-canonical activation of NFκB transcription factors

Figure 5

Analysis of NFκB binding sites in TNFα-stimulated cells by chromatin immunoprecipitation. T98G cells proliferating in serum-containing media were either left untreated or stimulated with TNFα for 15, 30 or 60 minutes. Chromatin fragments were immunoprecipitated with anti-p65 antibody and quantified by real-time PCR. Data are presented as the percentage of input and are the means of 2 independent experiments ± S.E. β-globin was used as the negative control.

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