Figure 1From: Phosphatase inhibitor 2 promotes acetylation of tubulin in the primary cilium of human retinal epithelial cellsI-2 localization by specific antibody staining in human ARPE-19 cells. (A) Extracts from confluent ARPE-19 cells and MCF-7 cells were subjected to Western blotting using affinity-purified sheep antibody specific for human I-2. This antibody only recognized I-2, and its phosphorylated form (minor upper band) in both cell lines. (B) Confluent ARPE-19 cells were fixed, and stained with anti-I-2 antibody as described under Methods. Compressed Z-stack of optical sections from laser confocal microscopy is shown. Scale bar = 20 micron.Back to article page