Silencing cytokeratin 18 gene inhibits intracellular replication of Trypanosoma cruzi in HeLa cells but not binding and invasion of trypanosomes

Table 1 RNAi – medited knockdown of CK18 inhibits intracellular multiplication of amastigotes of Y or CL-Brener strains

^a HeLa cells were transiently transfected with CK18 or control RNAi 24 h earlier as described in Methods section. These cells were infected with culture trypomastigotes of Y or CL-Brener strain using the parasite per cell ratio of 10:1 or 5:1 respectively. The number of intracellular amastigotes was estimated 48 h post-infection and the results are expressed as average of triplicates ± SD. This experiment was performed 3 times with similar results.
^b The number of amastigotes in cultures containing medium or RNAi control are lower than the number of amastigotes in cultures containing Medium with control RNAi (P < 0.01 in all cases, One Way Anova).

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