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Table 1 RNAi – medited knockdown of CK18 inhibits intracellular multiplication of amastigotes of Y or CL-Brener strains

From: Silencing cytokeratin 18 gene inhibits intracellular replication of Trypanosoma cruzi in HeLa cells but not binding and invasion of trypanosomes

Parasite Strain % of infected cell Amastigotes/100 cells
  Medium CK18 RNAi a CTR RNAi a Medium CK18 RNAi a CTR RNAi a
Y 32.0 ± 4.95 30.0 ± 3.53 28.0 ± 2.83 578 ± 11.36 142 ± 20.66 b 497 ± 16.04
CL-Brener 33.0 ± 2.0 35.0 ± 4.16 33.0 ± 4.04 411 ± 14.57 176 ± 18.55 b 406 ± 16.92
  1. a HeLa cells were transiently transfected with CK18 or control RNAi 24 h earlier as described in Methods section. These cells were infected with culture trypomastigotes of Y or CL-Brener strain using the parasite per cell ratio of 10:1 or 5:1 respectively. The number of intracellular amastigotes was estimated 48 h post-infection and the results are expressed as average of triplicates ± SD. This experiment was performed 3 times with similar results.
  2. b The number of amastigotes in cultures containing medium or RNAi control are lower than the number of amastigotes in cultures containing Medium with control RNAi (P < 0.01 in all cases, One Way Anova).