Figure 1

TLRR is a leucine-rich repeat protein. (A) The DNA sequence of the interacting clone (IC#2) is shown with the translated sequence above the nucleotide sequence. The leucine-rich repeats (LRRs) are indicated with solid underlining and the peptide used for preparation of antibody indicated with dashed underlining. The putative PP1 binding site is boxed. (B) The TLRR repeats are aligned with the LRR consensus sequence for the sds22 subfamily of LRR proteins. (C) An alignment between TLRR and Ppp1r7 is shown with the LRRs indicated with overlines (TLRR) and underlines (Ppp1r7). Multiple sequence alignment was done using ClustalW 1.82 and the results displayed using BOXSHADE 3.31. LRRs in TLRR and Ppp1r7 were identified using the Pfam protein domain database [35].