TLRR is expressed primarily in testis. (A) A multiple tissue northern blot was hybridized with radiolabeled probe specific for the TLRR mRNA as described in Methods. Each lane contains 2 μg mouse poly(A+) RNA separated on a denaturing formaldehyde 1.0% agarose gel and transferred to nylon. Lanes 1–8 contain: H, heart; B, whole brain; S, spleen; Lu, lung; Li, liver; SM, skeletal muscle; K, kidney; T, testis. The migration and size of RNA markers is shown on the left. (B and C) Total protein (75 μg) from each of the indicated tissues was separated by PAGE, transferred to membrane, and incubated with the affinity purified TLRR antibody (B), or preimmune serum (C). Lanes 1–8 in both B and C contain: T, testis; S, spleen; B, whole brain; H, heart; Lu, lung; Li, liver; P, pancreas; Sp, epidydimal sperm.