Figure 6

High contrast images of differentiated HUVEC in the TLA assay by Differential Interference Contrast (DIC) and confocal fluorescence microscopy. HUVEC stimulated to form tube-like structures with 25 ng/ml VEGF on a 35 mm glass-bottom dish covered with 10 μl matrix is compatible with DIC (x20 objective) (A) and confocal microscopy (x63 objective); Scale bar = 25 μM (B). Mitochondria (green) were stained using MitoTraker green (200nM). For 3D imaging (C) of the mitochondrial network, z-stacks were obtained at x40 magnification and re-constructed using Volocity software.