A morphologic and semi-quantitative technique to analyze synthesis and release of specific proteins in cells

Table 3 OD value of secreted IgG detected by ELISA in cell culture supernatant

	Normal culture condition	HPG	HPG plus anisomycin	Normal culture medium	PBS
OD value	0.078	0.1473	0.0799	0.0800	0.0820

Newly synthesized IgG in culture supernatant was detected by non-radioactive metabolic method combined with an adapted ELISA method. Triplicate for each sample. n = triplicate.

ISSN: 2661-8850