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Table 3 OD value of secreted IgG detected by ELISA in cell culture supernatant

From: A morphologic and semi-quantitative technique to analyze synthesis and release of specific proteins in cells

  Normal culture condition HPG HPG plus anisomycin Normal culture medium PBS
OD value 0.078 0.1473 0.0799 0.0800 0.0820
  1. Newly synthesized IgG in culture supernatant was detected by non-radioactive metabolic method combined with an adapted ELISA method. Triplicate for each sample. n = triplicate.