Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells

Paola Romina Amable; Marcus Vinicius Telles Teixeira; Rosana Bizon Vieira Carias; José Mauro Granjeiro; Radovan Borojevic

doi:10.1186/s12860-014-0046-0

BMC Molecular and Cell Biology

Table 2 Protein secretion (expressed as mean ± standard deviation) of adipose tissue- (AT-), bone marrow- (BM-) and Wharton’s Jelly-mesenchymal stromal cells (WJ-MSC) when grown in low glucose media supplemented with 10% fetal bovine serum

From: Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells

	BM-MSC	AT-MSC	WJ-MSC
Chemokines (pg/10 ⁶ cells/day)
IP-10	0.8 ± 0.3 ^a	nd	3.5 ± 0.4 ^a
MCP-1	727.5 ± 86.5 ^a	336.2 ± 42.2 ^b	551.5 ± 51.6 ^a,b
Pro-inflammatory cytokines (pg/10 ⁶ cells/day)
IL-6	46.6 ± 2.8 ^a	31.2 ± 4.3 ^a	146.0 ± 31.6 ^b
IL-7	102.4 ± 57.8 ^a	0.13 ± 0.19 ^a	0.07 ± 0.10 ^a
IL-8	628.3 ± 8.0 ^a	63.7 ± 2.7 ^a	4,466.3 ± 634.6 ^b
IL-12	41.5 ± 7.6 ^a	1.6 ± 2.3 ^a	nd
Anti-inflammatory cytokines (pg/10 ⁶ cells/day)
IL-1ra	30.00 ± 0.01 ^a	nd	205.3 ± 28.7 ^a
Angiogenic factors (pg/10 ⁶ cells/day)
Angiogenin	3,054.7 ± 74.2 ^a	995.3 ± 34.9 ^b	517.9 ± 5.3 ^c
Thrombospondin-2	79,967.7 ± 149.8 ^a	35,564.0 ± 1,644.6 ^b	155,582.9 ± 6,467.8 ^c
PLGF	102.4 ± 2.1 ^a	12.5 ± 1.0 ^a	nd
aFGF	65.7 ± 12.4 ^a	6.9 ± 6.5 ^b	14.0 ± 2.6 ^b
VEGF-D	24.80 ± 0.01	nd	nd
Endostatin	1,322.3 ± 24.7 ^a	1,270.4 ± 21.6 ^a	2,343.3 ± 68.7 ^b
Angiopoietin-1	129,196.1 ± 8,604.8 ^b	12,502.3 ± 405.8 ^a	17,114.5 ± 51.9 ^a
VEGF	3,431.8 ± 180.9 ^b	392.4 ± 24.4 ^a	3.4 ± 0.8 ^a
Growth factors (pg/10 ⁶ cells/day)
TGF-β1	4,051.7 ± 0.0 ^a	1,844.7 ± 205.9 ^b	1,622.6 ± 51.1 ^b
TGF-β2	178.1 ± 1.1 ^a	17.7 ± 3.3 ^b	13.7 ± 3.7 ^b
HGF	268.0 ± 8.4 ^a,b	44.8 ± 8.6 ^a	314.0 ± 86.6 ^b
G-CSF	nd	nd	52.6 ± 5.9
PDGF-AA	7.9 ± 1.5 ^a	2.5 ± 4.7 ^a	6.9 ± 1.7 ^a
Extracellular matrix proteins (pg/10 ⁶ cells/day)
Collagen I	76.6 ± 6.0 ^a	313.2 ± 53.0 ^b	15.6 ± 0.8 ^a
Collagen II	2,210.4 ± 911.3	nd	nd
Collagen III	12,332.2 ± 0.0 ^a	17,164.5 ± 689.8 ^a	38,914.8 ± 477.9 ^b
Collagen IV	116.5 ± 2.0 ^a	51.9 ± 4.2 ^b	nd
Elastin	959.3 ± 236.4 ^a	300.1 ± 6.5 ^b	nd
Fibronectin	nd	1.4 ± 1.2	nd
Heparan sulfate	235.6 ± 111.0 ^a	27.5 ± 233.0 ^a	nd
Laminin	95.5 ± 14.0 ^a	42.2 ± 15.2 ^a	nd
Aggrecan	2.7 ± 0.1 ^a	0.2 ± 0.4 ^a	nd
Matrix metalloproteinases (pg/10 ⁶ cells/day)
MMP1	149.6 ± 0.0 ^a,b	284.2 ± 11.0 ^a	364.0 ± 36.9 ^b
MMP3	1,235.1 ± 0.0 ^a	17,387.1 ± 3,213.2 ^b	684.2 ± 49.9 ^a
MMP7	675.6 ± 0.0 ^a,b	102.9 ± 26.7 ^a	35.5 ± 15.7 ^b
MMP8	527.0 ± 152.2 ^a	nd	10.6 ± 157.3 ^b
MMP13	24,201.6 ± 124.7 ^a	22.5 ± 0.0 ^b	nd

aFGF: acidic fibroblast growth factor; AT-MSC: adipose tissue-derived MSC; BM-MSC: bone marrow-derived MSC; GCS-F: granulocyte-colony stimulating factor; HGF: hepatocyte growth facctor; IL: interleukin; IP-10: interferon-gamma induced protein 10; MCP-1: monocyte chemotactic protein 1; MMP: metalloproteinase; nd: not determined; PDGF-AA: platelet derived growth factor AA; pg: picogram; PLGF: placental growth factor; TGF: transforming growth factor; VEGF: vascular endothelial growth factor; WJ-MSC: Wharton’s Jelly-derived MSC.
Protein concentration is expressed in pg secreted per million cells and per day. ANOVA and Bonferroni tests (α = 0.5, n = 3) were applied for each quantified factor in order to determine significant differences between all three MSC. For a specific protein; different letters (superscript letters a, b and c) mean statistically different expression levels.

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