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Table 2 Protein secretion (expressed as mean ± standard deviation) of adipose tissue- (AT-), bone marrow- (BM-) and Wharton’s Jelly-mesenchymal stromal cells (WJ-MSC) when grown in low glucose media supplemented with 10% fetal bovine serum

From: Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells

  BM-MSC AT-MSC WJ-MSC
Chemokines (pg/10 6 cells/day)
IP-10 0.8 ± 0.3 a nd 3.5 ± 0.4 a
MCP-1 727.5 ± 86.5 a 336.2 ± 42.2 b 551.5 ± 51.6 a,b
Pro-inflammatory cytokines (pg/10 6 cells/day)
IL-6 46.6 ± 2.8 a 31.2 ± 4.3 a 146.0 ± 31.6 b
IL-7 102.4 ± 57.8 a 0.13 ± 0.19 a 0.07 ± 0.10 a
IL-8 628.3 ± 8.0 a 63.7 ± 2.7 a 4,466.3 ± 634.6 b
IL-12 41.5 ± 7.6 a 1.6 ± 2.3 a nd
Anti-inflammatory cytokines (pg/10 6 cells/day)
IL-1ra 30.00 ± 0.01 a nd 205.3 ± 28.7 a
Angiogenic factors (pg/10 6 cells/day)
Angiogenin 3,054.7 ± 74.2 a 995.3 ± 34.9 b 517.9 ± 5.3 c
Thrombospondin-2 79,967.7 ± 149.8 a 35,564.0 ± 1,644.6 b 155,582.9 ± 6,467.8 c
PLGF 102.4 ± 2.1 a 12.5 ± 1.0 a nd
aFGF 65.7 ± 12.4 a 6.9 ± 6.5 b 14.0 ± 2.6 b
VEGF-D 24.80 ± 0.01 nd nd
Endostatin 1,322.3 ± 24.7 a 1,270.4 ± 21.6 a 2,343.3 ± 68.7 b
Angiopoietin-1 129,196.1 ± 8,604.8 b 12,502.3 ± 405.8 a 17,114.5 ± 51.9 a
VEGF 3,431.8 ± 180.9 b 392.4 ± 24.4 a 3.4 ± 0.8 a
Growth factors (pg/10 6 cells/day)
TGF-β1 4,051.7 ± 0.0 a 1,844.7 ± 205.9 b 1,622.6 ± 51.1 b
TGF-β2 178.1 ± 1.1 a 17.7 ± 3.3 b 13.7 ± 3.7 b
HGF 268.0 ± 8.4 a,b 44.8 ± 8.6 a 314.0 ± 86.6 b
G-CSF nd nd 52.6 ± 5.9
PDGF-AA 7.9 ± 1.5 a 2.5 ± 4.7 a 6.9 ± 1.7 a
Extracellular matrix proteins (pg/10 6 cells/day)
Collagen I 76.6 ± 6.0 a 313.2 ± 53.0 b 15.6 ± 0.8 a
Collagen II 2,210.4 ± 911.3 nd nd
Collagen III 12,332.2 ± 0.0 a 17,164.5 ± 689.8 a 38,914.8 ± 477.9 b
Collagen IV 116.5 ± 2.0 a 51.9 ± 4.2 b nd
Elastin 959.3 ± 236.4 a 300.1 ± 6.5 b nd
Fibronectin nd 1.4 ± 1.2 nd
Heparan sulfate 235.6 ± 111.0 a 27.5 ± 233.0 a nd
Laminin 95.5 ± 14.0 a 42.2 ± 15.2 a nd
Aggrecan 2.7 ± 0.1 a 0.2 ± 0.4 a nd
Matrix metalloproteinases (pg/10 6 cells/day)
MMP1 149.6 ± 0.0 a,b 284.2 ± 11.0 a 364.0 ± 36.9 b
MMP3 1,235.1 ± 0.0 a 17,387.1 ± 3,213.2 b 684.2 ± 49.9 a
MMP7 675.6 ± 0.0 a,b 102.9 ± 26.7 a 35.5 ± 15.7 b
MMP8 527.0 ± 152.2 a nd 10.6 ± 157.3 b
MMP13 24,201.6 ± 124.7 a 22.5 ± 0.0 b nd
  1. aFGF: acidic fibroblast growth factor; AT-MSC: adipose tissue-derived MSC; BM-MSC: bone marrow-derived MSC; GCS-F: granulocyte-colony stimulating factor; HGF: hepatocyte growth facctor; IL: interleukin; IP-10: interferon-gamma induced protein 10; MCP-1: monocyte chemotactic protein 1; MMP: metalloproteinase; nd: not determined; PDGF-AA: platelet derived growth factor AA; pg: picogram; PLGF: placental growth factor; TGF: transforming growth factor; VEGF: vascular endothelial growth factor; WJ-MSC: Wharton’s Jelly-derived MSC.
  2. Protein concentration is expressed in pg secreted per million cells and per day. ANOVA and Bonferroni tests (α = 0.5, n = 3) were applied for each quantified factor in order to determine significant differences between all three MSC. For a specific protein; different letters (superscript letters a, b and c) mean statistically different expression levels.