Figure 2
Stability and reduction possibilities of DHF ricin and IHF ricin. (A) Coomasie Blue-stained 12% SDS/PAGE gels showing the effect of the incubation of 500 ng of wild-type ricin A-chain, RTA wt, and modified ricin A-chains: RTADHF and RTAIHF, with increasing concentrations of trypsin. Digestion patterns for denatured forms of RTA are also shown. (B) Coomasie Blue-stained 12% SDS/PAGE gels showing the effect of increasing concentrations of dithriothreitol (DTT) on the reduction of native and denatured forms of wild-type holotoxin, DHF holotoxin and IHF holotoxin.